ABSTRACT
To study the gene expression of cardiac mesenchymal cells in patients with type 2 diabetes mellitus (T2DM)based on a whole-genome high-throughput sequencing dataset,screen differentially expressed genes,analyze the genetics signature of cardiac mesenchymal cells in T2DM patients by bioinformatics analysis,and explore the environmental chemicals related to the key differentially expressed genes. The dataset GSE106177 was obtained from Gene Expression Omnibus (GEO) database.The dataset was pre-processed and analyzed by Network Analyst,Cytoscape 3.7.1,String11.0,CTD,and HMDD for screening for differentially expressed genes,enrichment analysis,establishment of protein-protein interaction (PPI) networks,and screening for relevant environmental chemicals. The gene expression pattern of cardiac mesenchymal cells in T2DM patients was significantly different from that in the control group.There were 135 differentially expressed genes,of which 58 (42.96%) were up-regulated and 77 (57.04%) were down-regulated.The differentially expressed genes mainly participated in biological processes such as multicellular organism development,anatomical structure development,and system development and were mainly involved in hepatocellular carcinoma,Cushing's syndrome,and cholesterol metabolism.PPI network showed that UBC was the core protein node.The microRNA-Gene interaction network showed that seven microRNAs,represented by hsa-mir-8485,interacted with the differentially expressed genes.Key T2DM related genes such as UBC,DNER,and CNTN1 interacted with bisphenol A. The gene expression profile of cardiac mesenchymal cells markedly changes in T2DM patients,during which UBC may play an important biological role.Bisphenol A exposure may also affect the development and normal function of cardiac cells in T2DM patients.
ABSTRACT
Objective To investigate the therapeutic effect of neurotropin (NTP) combined with methylcobalamin for the treatment of residual neurological symptoms after surgery for degenerative lumbar disease (DLD).Methods A total of 96 cases with residual neurological symptoms after surgery for DLD from Jan.,2012 to Jun.,2015 were enrolled in this study and divided into three groups,32 cases in each group.The patients in group A were treated with methylcobalamin,the patients in group B were treated with NTP,and the patients in group C were treated with NTP + methylcobalamin.All the patients were treated for 2 weeks.The visual analogue scale (VAS) and oswestry disability index (ODI) were observed and documented at the beginning of the treatment,14 days,3 months,6 months and 12 months after the treatment,respectively.Results The VAS and ODI scores at 14 days,3 months,6 months and 12 months after the treatment in each group decreased obviously comparing with the scores before the treatment (P＜0.05).At each time point the VAS and ODI scores in group C＜group B＜group A (P＜0.05).The excellent rate at each time point in group C＞group B＞group A (P ＜ 0.05).Conclusions NTP is effective in the treatment of residual neurological symptoms and its therapeutic effects is better than methylcobalamin.The combined application of NTP and methylcobalamin is superior to the single application of NTP or methylcobalamin for the treatment of residual neurological symptoms after surgery for degenerative lumbar diseases.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the safety and therapeutic effects of monosegment pedicle instrumentation in treating incomplete thoracolumbar burst fracture.</p><p><b>METHODS</b>A retrospective analysis was conducted on 56 inpatients with incomplete thoracolumbar burst fracture (AO classification: A3.1 and A3.2) from April 2005 to January 2010. There were 28 cases were fixed with monosegment pedicle instrumentation (MSPI), 28 cases were fixed with short segment pedicle instrumentation (SSPI). The operative time, blood loss, visual analogue scale (VAS) and vertebral kyphotic angle (VK) before and after surgery were evaluated.</p><p><b>RESULTS</b>In the group of MSPI, the mean operative time was (93 ± 20) min; the intraoperative blood loss was (184 ± 64) ml; the VK angle was 17° ± 10° before operation, 7° ± 7° at one week after operation, and 10° ± 7° at latest follow-up; VAS score was 7.6 ± 1.5 before operation, 2.4 ± 0.8 at one week after operation, and 1.5 ± 0.9 at latest follow-up; no adjacent segment degeneration was found. In the group of SSPI, the operative time was (102 ± 30) min; the intraoperative blood loss was (203 ± 88) ml; the VK angle was 17° ± 9° before operation, 7° ± 7° at one week after operation, and 8° ± 5° at latest follow-up; VAS score was 6.8 ± 1.3 before operation, 3.1 ± 0.5 at one week after operation, and 1.2 ± 0.7 at latest follow-up. One case of adjacent segment degeneration was found in 36 months after operation. There were no significantly statistical differences between two groups in operative time, blood loss, VAS score and VK angle before and after surgery (P > 0.05). The VAS score and VK angle at one week after surgery and latest follow-up all decreased obviously than preoperative ones in both groups (P < 0.05).</p><p><b>CONCLUSIONS</b>MSPI for incomplete thoracolumbar burst fracture is effective and safe. The operative blood loss, the mean operative time, the improvement of VAS score and the VK angle in group MSPI are equal to those in group SSPI.</p>